<br>Hi Kishore,<br><br>Yes, absolutely right. In my opinion, this is an "artistic" project (despite the use of advanced microscopy and image processing techniques).<br><br>Some effort is given to maintain a resemblance to an "original" object, but there can be no clear assertion of accuracy in the work. At the start, there are some distortions due to fixing and microtome sectioning of the samples. In an ideal world, each section would be a composition of the object of interest with a microscopic or nanoscopic grid that would provide a measure for these distortions. The use of a deformation registration is a poor attempt to "undo" these deformations. Of course, it assumes we begin with an object that has no deformations and that's just not possible in this project.<br>
<br>Even if the input data had perfect mapping to the original object of interest, the image processing would introduce some artifacts that propagate through the series. The order of the Bspline deformation is low (3) in an attempt to limit the deformations.<br>
<br>It might be ideal to constrain the alignment process within a short subset of the image series (maybe 3, 5, 7, or 9 images), with some kind of "sliding window" to move through the entire series (where the slide would involve some degree of window overlap). This may not be a strictly pair-wise image registration process (it may be an N-way registration) and it might occur best in a 3D method that was able to maintain a translation between consecutive images to maintain their separation in 3D space (the z-spacing). AFAIK, this "ambiguous" registration method is outside the design parameters of ITK (where registration puts any TWO images into the "same" space).<br>
<br>Any suggestions on how to do some kind of "sliding" window registration would be wondeful.<br><br>Take care,<br>Darren<br><br><br><br><div class="gmail_quote">On Tue, Oct 20, 2009 at 4:51 PM, Kishore Mosaliganti <span dir="ltr"><<a href="mailto:kishoreraom@gmail.com">kishoreraom@gmail.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">Hi,<br>
<br>