<br>HI Reinaldo,<br><br>Is your goal to segment and quantify shape and size of the droplets ?<br><br>If so, you may find useful the methods used for analyzing cells in<br>microscopy images.<br><br>In particular the ones described in the following Insight Journal papers<br>
(whose source code is available).<br><br>"Blob Segmentation":<br><a href="http://www.insight-journal.org/browse/publication/321">http://www.insight-journal.org/browse/publication/321</a><br><br>"Watersheds"<br>
<a href="http://www.insight-journal.org/browse/publication/92">http://www.insight-journal.org/browse/publication/92</a><br><br>"Consolidated Morphology"<br><a href="http://www.insight-journal.org/browse/publication/124">http://www.insight-journal.org/browse/publication/124</a><br>
<br>and related papers:<br><a href="http://www.insight-journal.org/browse/publication/642">http://www.insight-journal.org/browse/publication/642</a><br><a href="http://www.insight-journal.org/browse/publication/322">http://www.insight-journal.org/browse/publication/322</a><br>
<br><br>If your droplets are similar enough, and rotationally <br>symmetric, you could do pattern matching in the Fourier<br>domain, by computing the Fourier transform of your <br>large image, and the template, multiplying them in<br>
the Fourier domain, and then computing the inverse<br>Fourier transform of the product.<br><br>This will be the equivalent of compute correlation in <br>the spatial domain of the image.<br><br>You could do this with each one of the templates...<br>
and then count peaks in the output image by using<br>the LocalMaxima filter.<br><br><br> Regards,<br><br><br> Luis<br><br><br>------------------------------------------------------------------------<br><div class="gmail_quote">
On Wed, Jul 1, 2009 at 3:40 PM, Reinaldo <span dir="ltr"><<a href="mailto:rheineo@gmail.com">rheineo@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
<div style="text-align: left;" dir="ltr">Hi, Luis<br><br>thanks .. <br><br>I am analyzing images obtained on emulsions of an electron microscope, is composed of an emulsion droplets. <br><br>
I need to consider the drops as I have in some small images (templates)
shows that the emulsion, the drops are for different kinds and sizes,
the intention is to know how many of each type are in the picture. <br><br> Templates are greater than 10x10 pixel <br> The original image has a pixel size of 576x720</div><br> Thanks<br>-- <br><font color="#888888">REINALDO<br>
</font></blockquote></div><br>